Mailing Address:
University of California, Berkeley
Dept. of Integrative Biology
3060 VLSB #3140
Berkeley, CA 94720-3140
Lab TEL (510) 643-4201
FAX (510) 643-5022
Email: ekwan[at]berkeley.edu
University of California, Berkeley
Dept. of Integrative Biology
3060 VLSB #3140
Berkeley, CA 94720-3140
Lab TEL (510) 643-4201
FAX (510) 643-5022
Email: ekwan[at]berkeley.edu
Research Summary
Currently, I am responsible for the production of monoclonal and polyclonal antibodies against protein antigens of developmentally important genes in the amphipod crustacean Pahyale hawaiensis, and for maintaining the lab’s inventory of monoclonal antibodies to Drosophila and grasshopper proteins produced over the years.
Routinely, antigens are prepared by expressing gene sequences of interest as fusion proteins in bacteria. Vectors producing GST, TrpE, or 6xHistidine fusion proteins have been employed. Thus far, we have successfully raised polyclonal antibodies against Parhyale proteins encoded by orthologs of the Drosophila pair-rule genes even-skipped and Pax3/7. Polyclonal antibodies against odd-skipped and odd-paired are being developed. I am also working with Sandip Chatterjee to produce a fusion protein for generating antiserum to the Parhyale Hox protein, Ubx.
These antibodies are important tools for characterizing the dynamic expression patterns of these genes in the developing embryo. Furthermore, they allow us to assess the effects of gene knockdown and misexpression experiments.
Publications
Zhang, S., Dailey, G.M., Kwan, E., Glasheen, B.M., Sroga. G.E., and Page-McCaw, A. (2006). An MMP Liberates the Ninjurin A Ectodomain to signal a Loss of Cell Adhesion. Genes & Dev. 20, 1899-1910.
Brodsky, M.H., Nordstrom, W., Tsang, G., Kwan, E., and Rubin, G.M. (2000). Drosophila p53 Binds a Damage Response Element at the reaper Locus. Cell 101, 103-113.
Therrien, M., Wong, A.L., Kwan, E., and Rubin, G.M. (1999). Functional Analysis of CNK in Ras Signaling. PNAS 96, 13259-13263.
Tang, A.H., Neufeld, T.P., Kwan, E., and Rubin, G.M. (1997). PHYL Acts to Down-Regulate TTK88, a Transcriptional Repressor of Neuronal Cell Fates, by a SINA-Dependent Mechanism. Cell 90, 459-467.
Fairchild, S.S., Shannon, K., Kwan, E, and Mishell, R.I. (1984). T Cell-derived Glucosteroid Response Modifying factor (GRMFT): A Unique Lymphokine Made by Normal T Lymphocytes and a T Cell Hybridoma. J. Immunology 132, 821-827.
Slomich, M., Kwan, E., and Mishell, R.I. (1980). Biology of Accessory Cerlls Required for antigen-specificd T Lymphocyte Proliferation. Fed. Proc., 39:912.
Kwan, E., Mishell, R.I., and Mishell, B.B. (1980). “Cell Separation: Rossette Removal by Agglutination,” Selected Methods in Cellular Immunology, San Francisco: W.H. Freeman and Company, pp. 217-218.
Kwan, E., Mishell, R.I., and Mishell, B.B. (1980). “Cell Separation: Fc and Complement Receptors,” Selected Methods in Cellular Immunology, San Francisco: W.H. Freeman and Company, pp. 219-224.
Slomich, M., Kwan, E., Wofsy, L., and Henry, C. (1980). “Cell Separation: Hapten-sandwich Rosetting,” Selected Methods in Cellular Immunology, San Francisco: W.H. Freeman and Company, pp. 212-216.
Work Experience
2006-Present Research Technician II, Nipam H. Patel Laboratory, Howard Hughes
Medical Institute, University of California, Berkeley, CA
1995-2006 Research Technician II, Gerald Rubin Laboratory, Howard
Hughes Medical Institute, University of California, Berkeley, CA
1992-1994 Research Associate III, Metabolex, Inc. Hayward, CA
1988-1991 Laboratory Technician, Research Institute, Children’s Hospital, Oakland, CA
1986-1988 Biological Laboratory Technician, USDA-Agricultural Research Station, Salinas, CA
1975-1986 Staff Research Associate I to III, University of California, Berkeley, CA
Education
B.S. in Dietetics, 1975. University of California, Berkeley
Currently, I am responsible for the production of monoclonal and polyclonal antibodies against protein antigens of developmentally important genes in the amphipod crustacean Pahyale hawaiensis, and for maintaining the lab’s inventory of monoclonal antibodies to Drosophila and grasshopper proteins produced over the years.
Routinely, antigens are prepared by expressing gene sequences of interest as fusion proteins in bacteria. Vectors producing GST, TrpE, or 6xHistidine fusion proteins have been employed. Thus far, we have successfully raised polyclonal antibodies against Parhyale proteins encoded by orthologs of the Drosophila pair-rule genes even-skipped and Pax3/7. Polyclonal antibodies against odd-skipped and odd-paired are being developed. I am also working with Sandip Chatterjee to produce a fusion protein for generating antiserum to the Parhyale Hox protein, Ubx.
These antibodies are important tools for characterizing the dynamic expression patterns of these genes in the developing embryo. Furthermore, they allow us to assess the effects of gene knockdown and misexpression experiments.
Publications
Zhang, S., Dailey, G.M., Kwan, E., Glasheen, B.M., Sroga. G.E., and Page-McCaw, A. (2006). An MMP Liberates the Ninjurin A Ectodomain to signal a Loss of Cell Adhesion. Genes & Dev. 20, 1899-1910.
Brodsky, M.H., Nordstrom, W., Tsang, G., Kwan, E., and Rubin, G.M. (2000). Drosophila p53 Binds a Damage Response Element at the reaper Locus. Cell 101, 103-113.
Therrien, M., Wong, A.L., Kwan, E., and Rubin, G.M. (1999). Functional Analysis of CNK in Ras Signaling. PNAS 96, 13259-13263.
Tang, A.H., Neufeld, T.P., Kwan, E., and Rubin, G.M. (1997). PHYL Acts to Down-Regulate TTK88, a Transcriptional Repressor of Neuronal Cell Fates, by a SINA-Dependent Mechanism. Cell 90, 459-467.
Fairchild, S.S., Shannon, K., Kwan, E, and Mishell, R.I. (1984). T Cell-derived Glucosteroid Response Modifying factor (GRMFT): A Unique Lymphokine Made by Normal T Lymphocytes and a T Cell Hybridoma. J. Immunology 132, 821-827.
Slomich, M., Kwan, E., and Mishell, R.I. (1980). Biology of Accessory Cerlls Required for antigen-specificd T Lymphocyte Proliferation. Fed. Proc., 39:912.
Kwan, E., Mishell, R.I., and Mishell, B.B. (1980). “Cell Separation: Rossette Removal by Agglutination,” Selected Methods in Cellular Immunology, San Francisco: W.H. Freeman and Company, pp. 217-218.
Kwan, E., Mishell, R.I., and Mishell, B.B. (1980). “Cell Separation: Fc and Complement Receptors,” Selected Methods in Cellular Immunology, San Francisco: W.H. Freeman and Company, pp. 219-224.
Slomich, M., Kwan, E., Wofsy, L., and Henry, C. (1980). “Cell Separation: Hapten-sandwich Rosetting,” Selected Methods in Cellular Immunology, San Francisco: W.H. Freeman and Company, pp. 212-216.
Work Experience
2006-Present Research Technician II, Nipam H. Patel Laboratory, Howard Hughes
Medical Institute, University of California, Berkeley, CA
1995-2006 Research Technician II, Gerald Rubin Laboratory, Howard
Hughes Medical Institute, University of California, Berkeley, CA
1992-1994 Research Associate III, Metabolex, Inc. Hayward, CA
1988-1991 Laboratory Technician, Research Institute, Children’s Hospital, Oakland, CA
1986-1988 Biological Laboratory Technician, USDA-Agricultural Research Station, Salinas, CA
1975-1986 Staff Research Associate I to III, University of California, Berkeley, CA
Education
B.S. in Dietetics, 1975. University of California, Berkeley
NIPAM H. PATEL